how to read a refractometer for total protein|total solids range for dogs : ODM Quantitation of protein in cerebrospinal fluid, urine and other low-protein fluids requires more sensitive techniques than either the Biuret or refractometer method. Protein in these fluids can be measured more accurately with precipitation or dye-binding methods.
Prepare saturated ammonium sulfate ( (NH 4) 2 SO 4, ~ 1kg/liter ), autoclave. 2. Add equal volume of saturated ammonium sulfate to antibody sample. Stir at 4 °C overnight. 3. Spin .
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Total protein by refractometer measurement. Many refractometers, such as the one depicted here, has specific gravity scales for urine in cats (left scale) and dogs (middle scale). .Plasma protein assays can be performed either using a refractometer or an automated biochemistry analyser. Total plasma protein can be measured using the plasma present in a .
Read the refractometer to determine the plasma protein. If the distinction between the blue and the white is blurry this can mean that there is insufficient plasma on the reading plate. Try pressing the lid down firmly as this can make the line clearer. Refractometers may have 2 . Total protein by refractometer measurement. Many refractometers, such as the one depicted here, has specific gravity scales for urine in cats (left scale) and dogs (middle scale). The third scale is for total protein measurement on serum, plasma or body cavity fluids.Plasma protein assays can be performed either using a refractometer or an automated biochemistry analyser. Total plasma protein can be measured using the plasma present in a capillary tube or sample container that has been centrifuged.Quantitation of protein in cerebrospinal fluid, urine and other low-protein fluids requires more sensitive techniques than either the Biuret or refractometer method. Protein in these fluids can be measured more accurately with precipitation or dye-binding methods.
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Total solids/protein. This is measured by refractometer and actually measures refractive index versus total protein. It is used as an estimate for total protein (which are the constituents of plasma that have the most effect on the refractive index).The use of refractometry is valid for providing an estimate of plasma protein concentration, since protein is the major determinant of refractive index of plasma. It is not, however, the only contributor. All other dissolved solids (glucose, urea, cholesterol, etc) also make a contribution.Refractometers are ubiquitously used in veterinary clinics to provide rapid, easy, inexpensive assessment of protein concentration in plasma and other fluids. Total protein determination using a refractometer is a quick, inexpensive, and extremely valuable in-house test. Measuring the plasma total protein value is necessary to fully interpret the packed cell volume (PCV), complete blood count (CBC), and urinalysis findings.
The most commonly used method for measuring serum total protein is the biuret reaction (see Total Protein on CD-ROM). The reaction is based on the color formed when copper complexes with the peptide bond.This clip demonstrates how to measure plasma total protein using a refractometer and a centrifuged capillary tube of EDTA blood.
Read the refractometer to determine the plasma protein. If the distinction between the blue and the white is blurry this can mean that there is insufficient plasma on the reading plate. Try pressing the lid down firmly as this can make the line clearer. Refractometers may have 2 . Total protein by refractometer measurement. Many refractometers, such as the one depicted here, has specific gravity scales for urine in cats (left scale) and dogs (middle scale). The third scale is for total protein measurement on serum, plasma or body cavity fluids.Plasma protein assays can be performed either using a refractometer or an automated biochemistry analyser. Total plasma protein can be measured using the plasma present in a capillary tube or sample container that has been centrifuged.Quantitation of protein in cerebrospinal fluid, urine and other low-protein fluids requires more sensitive techniques than either the Biuret or refractometer method. Protein in these fluids can be measured more accurately with precipitation or dye-binding methods.
Total solids/protein. This is measured by refractometer and actually measures refractive index versus total protein. It is used as an estimate for total protein (which are the constituents of plasma that have the most effect on the refractive index).The use of refractometry is valid for providing an estimate of plasma protein concentration, since protein is the major determinant of refractive index of plasma. It is not, however, the only contributor. All other dissolved solids (glucose, urea, cholesterol, etc) also make a contribution.
Refractometers are ubiquitously used in veterinary clinics to provide rapid, easy, inexpensive assessment of protein concentration in plasma and other fluids.
Total protein determination using a refractometer is a quick, inexpensive, and extremely valuable in-house test. Measuring the plasma total protein value is necessary to fully interpret the packed cell volume (PCV), complete blood count (CBC), and urinalysis findings. The most commonly used method for measuring serum total protein is the biuret reaction (see Total Protein on CD-ROM). The reaction is based on the color formed when copper complexes with the peptide bond.
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how to read a refractometer for total protein|total solids range for dogs